E.J. Ehrhart

Biography of Investigator:
Midwestern raised and educated. Undergraduate studies and professional Veterinary Medicine degree were completed at the University of Missouri- Columbia. I was in private practice for 4 years on the east coast and later in St Louis before pursuing graduate studies in Veterinary Pathology training. My anatomic pathology training was completed at CSU in a combined program with graduate studies for a Ph.D. Always having an interest in cancer and its effects on the body, my dissertation work was funded by a training grant through NASA on the effects of extended space travel and radiation exposure carcinogenic effects on mammary tissue. I completed my degree and accepted a faculty position at University of IL- Urbana in diagnostic pathology. I returned to CSU to be a member of the Animal Cancer Center when it was just opening it doors. I enjoy the dynamic team approach that takes cancer research from the bench to the patient.

Classes Taught/Currently Teaching:

• MIP555 Principles and Mechanisms of Disease: cancer pathology
• R580 Cancer Genetics
• R581 Cancer Biology
• VM640 Biology of Disease I: disturbances of growth and cancer
• VM742 Biology of Disease III
• PA778 Pathobiology of Laboratory Animals- Hamster & Gerbil
• PA796V-004 Independent study-Ophtho pathology

Research Focus:
The Molecular Pathology Laboratory at Colorado State University’s Animal Cancer Center has two functions, independent research and pathology support for research projects.

Independent research is driven by  strong interests in oncological pathology with corresponding strong backgrounds in multiple aspects of oncology including cancer proteomics, angiogenesis, carcinogenesis and tumor immunology.  Brad Charles, the laboratory manager has a background in cell and molecular biology with a strong interest in oncology, pathology, and immunology.

The pathology support core seeks to provide a variety of services, advice, and consultation to the Animal Cancer Center and to the whole of Colorado State University.  The services listed below are the heart of what the core offers but the mission of the core also includes supporting the Cancer Center through streamlining of pathology workflow, development of new techniques and pathology troubleshooting.  The core is available for help with any issue and offers the below services as piecemeal or is available to manage projects from necropsy all the way to data analysis.

Core services:

• Advice and consultation
• Necropsy, dissection and trimming
• Sample preparation and submission including decalcification and trimming
• Immunohistochemistry new protocol development
• Immunohistochemistry
• Immunofluorescence
• Western Blot/Dot Blot including quantification
• Image collection and digital pathology
• Image analysis
• Image analysis training and consultation
• Data collection and interpretation
• Histopathology

Future Direction:
The Molecular Pathology laboratory is actively completing necessary arrangements to be GLP compliant in sample dissection and processing, histopathology and immunohistochemistry.

Name of Laboratory:
ACC Molecular Pathology Laboratory

Laboratory Personnel:
Brad Charles - Laboratory Coordinator

List of Major Laboratory Equipment:

1. Ventanna Discovery Automated Stainer for immunohistochemistry and in situ hybridization
2. 1D and 2D gel electrophoresis
3. Zeiss Automated microscope with image analysis and epifluorescence

Current Work/Projects:

1. E.J. Ehrhart (principal investigator). 2-Deoxy-D-Glucose as a Therapy for Hypoxic Tumor Cells in Canine Osteosarcoma.
2. Susan Kraft (Primary investigator). Role: co-investigator. Metabolic profiling of canine lymphoma by nuclear magnetic resonance spectroscopy. Morris Animal Foundation.
3. Lana, Susan E. (Primary PI). Role: co-investigator. Provoking Antitumor Immune Responses with FAS Ligand

Publications:
J.L. Traub Dargatz, J.W. Schlipf, L. Vap, E.J. Ehrhart. Challenging cases in internal medicine: What's your diagnosis?. Veterinary Medicine. 2:100 104, 1994.

J.L. Traub Dargatz, J.W. Schlipf, E. Atwell, D.G. Bennet, R.L. Jones, E.J. Ehrhart, P.C. Schulteiss. Aeromonas hydrophila  septic arthritis in a neonatal foal. Equine Practice. 16(2):15 17, 1994.

N. Pirkey Ehrhart, S.J. Withrow, R.C. Straw, E.J. Ehrhart, R.L. Page, H.L. Hottinger, K.A. Hahn, W.B. Morrison, M.R. Albrecht, C.S. Hedlund, A.S. Hammer, D.L. Holmberg, A.S. Moore, R.R. King, J.S. Klausner. Primary rib tumors in 54 dogs. Journal of the American Animal Hospital Association. 31(1):65 69,1995.

D.A. Degner, M.S. Degner, E.J. Ehrhart. Palatine tonsil cyst in a dog. Journal of the American Veterinary Medical Association. 204(7):1041 1042,1994.

B.E. Powers, P.J. Hoopes, E.J. Ehrhart. Tumor Diagnosis, Grading, and Staging. In Seminars in Veterinary Medicine and Surgery. (Small Animal). 10(3):158 167,1995.

M.H. Barcellos Hoff, E.J. Ehrhart, M. Kalia, R. Jirtle, K. Flanders, M.L.Tsang. Immunohistochemical detection of active TGFß in situ using engineered tissue. American Journal of Pathology. 147(5):1228 1237, 1995.

E.J. Ehrhart, E.L. Gillette, M.H. Barcellos Hoff. Immunohistochemical evidence of rapid extracellular matrix remodeling following Fe particle irradiation of mouse mammary gland. Radiation Research. 145(2):157 162, 1996.

N. Ehrhart, S.J. Withrow, E.J. Ehrhart, J.H. Wimsatt. Pancreatic beta cell tumor in ferrets: 20 cases (1986-1994).  Journal of the American Veterinary Medical Association. 209(10); 15:1737 1740, 1996.

E.J. Ehrhart, P. Segarini, M. L. S. Tsang, A.G. Carroll, M.H. Barcellos Hoff. Latent transforming growth factor ß1 activation in situ: quantitative and functional evidence after low dose gamma irradiation. Federation of American Societies for Experimental Biology. (FASEB) 11(12):991 1002, 1997.

N. Ehrhart, W.S. Dernell, E.J. Ehrhart, J.M. Hutchison, E.B. Douple, J.H. Brekke, R.C. Straw, S.J. Withrow. The effects of a controlled release cisplatin delivery system (OPLAR Pt) after marginal resection of mouse mammary carcinoma. American Journal of Veterinary Research. 60(11):1347 1351, 1999.

S.S. Tunev, E.J. Ehrhart, H.E. Jensen, J.H. Foreman, R. A. Richter, J.B. Messick. Necrotizing mycotic vasculitis with cerebral infarction caused by Aspergillus niger in a horse with acute typhlocolitis. Veterinary Pathology. 36(4):347 51, 1999. §corresponding author.

L.L. Hoyer, J. Clevenger, J.E. Hecht, E.J. Ehrhart, F.M. Poulet. Detection of Als proteins on the cell wall of Candida albicans in murine tissues. Infectious Immunology. 67(8):4251 4255, 1999.

R.E. Guzman, E.J. Ehrhart, K. Wasson, J.J. Andrews. Primary hepatic hemangiosarcoma with pulmonary metastasis in a New Zealand white rabbit. Journal of Veterinary Diagnostic Investigation. 12:284-286, 2000. §corresponding author.

S.M. Aquino, R.E. Hamor, V.E. Valli, B.E. Kitchell, S.S. Tunev, K.L. Bailey, E.J. Ehrhart. Progression of an orbital T-cell rich B-cell lymphoma to a B-cell lymphoma in a dog. Veterinary Pathology. 37:465-469, 2000.

J.B. Messick, L.M. Berent, E.J. Ehrhart, C.C. Wasmer. Light and electron microscopic features of Eperythrozoon- like parasites in an opossum (Didelphis marsupialis). Journal of Zoology and Wildlife Medicine. 31(2):240-243, 2000.

B.R. Coolman, N. Ehrhart, J.L. Pijanowski, E.J. Ehrhart, S.L. Coolman.  Comparison of skin staples with sutures for anastomosis of the small intestine in dogs. Veterinary Surgery. 29(4):293 302, 2000.

M.J. Chladny, E.J. Ehrhart. Brain Vacuolization Induced by 70% Ethanol. Histologic; Technical Bulletin for Histotechnology. 3, 2000.

N. Ehrhart, E.J. Ehrhart, J.S. Willis,  D. Sisson, P. Constable, C.L. Greenfield, S. Manfra-Marretta, J. Hintermeister. Analysis of factors affecting survival in dogs with aortic body tumors. Veterinary Surgery. 31:44-8, 2002.

S. Sulaimon, B.E. Kitchell, E.J. Ehrhart. Immunohistochemical detection of melanoma specific antigens in spontaneous canine melanoma. Journal of Comparative Pathology, J Comp Pathol. 127 (2-3): 162-8, 2002.

J.M. Gordon, E.J. Ehrhart, D.D. Sisson, M.A. Jones. Juvenile hyperthyroidism in the cat.                    Journal of the American Animal Health Association. 39(1):67-71, 2003.

D.L. Hickman, K. Wasson, E.J. Ehrhart. Morbidity and mortality in a group of young guinea pigs. Subclinical hypovitaminosis C. Laboratory Animal. 32(9):23-25, 2003.

A.W. Biondo, E.J. Ehrhart, D.D. Sisson, B.J. Bulmer, H.S. Morais, P.F. Solter. Immunohistochemistry of atrial and brain natriuretic peptides in control cats and cats with hypertrophic cardiomyopathy. Vet Pathol. 40(5):501-506, 2003.

J. Liptak, W.S. Dernell, E.J. Ehrhart, S.A Rizzo, M.B. Rooney, S.J. Withrow. Retroperitoneal sarcoma in 14 dogs. Journal of the American Veterinary Medical Association., 224 (9): 1471-1477, 2004.

J.C. Whittemore, J.R. Gionfriddo, P.F. Seyn, E.J. Ehrhart. Indium-111 labeled vitamin B12 imaging of a ciliary adenoma with concurrent grade 2 soft tissue sarcoma of the leg in a Labrador Retriever dog. Vet Ophthalmology. 7(3):209-212, 2004.

M.N. Mullins, S.E. Lana, S.J. Withrow, W.S. Dernell, E.J.Ehrhart. Cycoloxygenase-2 expression in canine appendicular osteosarcomas. Journal of Veterinary Internal Medicine. 18(6): 859-865, 2004.

V. E. Valli, W. Vernau, L. P. DeLorimier, E. J. Ehrhart, J. Chladny, P. S. Graham Clonality and clinical behavior of canine indolent lymphoma. Veterinary and Comparative Oncology. 2(2)June: 110-111, 2004.

J.M. Liptak, S.J. Withrow, D.W. Macy, D.J. Frankel, E.J. Ehrhart. Metastatic synovial cell sarcoma in two cats. Veterinary and Comparative Oncology. 2(3): 164-170, 2004.

M.E. Hasson, G.S. Smith, R.S. Ott, D.B. Faulkner, L.D. Firkens, E.J. Ehrhart, D.S. Schaffer. Reversibility of the reproductive toxicity of gossypol in peripubertal bulls. Theriogenology. 61: 1171-1179, 2004.

L.B. Stokking, E.J.Ehrhart, C.A. Lichtensteiger, K.L. Campbell. Pigmented epidermal plaques in three dogs. Journal of the American Animal Hospital Association. 40(5):411-417, 2004.

A. Hoffman, T.Blocker, R. Dubielzig, E.J. Ehrhart. Feline periocular peripheral nerve sheath tumor: a case series. Veterinary Ophthalmology. 8(3):153-158, 2005.   

D. Kamstock, R. Fredrickson, E.J. Ehrhart. Lipid-rich carcinoma of the mammary gland in a cat. Veterinary Pathology. 42: 42:360-362,2005.

Dennis MM, Pearce LK, Norrdin RW, E.J. Ehrhart. Bacterial meningoencephalitis and ventriculitis due to migrating plant foreign bodies in three dogs. Vet Pathol. 2005 Nov;42(6):840-4.
 
Dennis MM, Ehrhart N, Duncan CG, Barnes AB, E.J. Ehrhart. Frequency of and risk factors associated with lingual lesions in dogs: 1,196 cases (1995-2004). J Am Vet Med Assoc. 2006 May 15;228(10):1533-7.
       
Green CB, Marretta SM, Cheng G, Faddoul FF, E.J. Ehrhart, Hoyer LL. RT-PCR analysis of Candida albicans ALS gene expression in a hyposalivatory rat model of oral candidiasis and in HIV-positive human patients. Med Mycol. 2006 Mar;44(2):103-11.

Dennis MM, Bennett N, E.J. Ehrhart. Gastric adenocarcinoma and chronic gastritis in two related Persian cats. Vet Pathol. 2006 May;43(3):358-62.     

Mullins MN, Dernell WS, Withrow SJ, E.J. Ehrhart, Thamm DH, Lana SE. Evaluation of prognostic factors associated with outcome in dogs with multiple cutaneous mast cell tumors treated with surgery with and without adjuvant treatment: 54 cases (1998-2004). J Am Vet Med Assoc. 2006 Jan 1;228(1):91-5.

Liptak JM, Kamstock DA, Dernell WS, E.J. Ehrhart, Rizzo SA, Withrow SJ.: Cranial mediastinal carcinoma in nine dogs. Vet Compar Oncol. Accepted, 3/2007. (In Press)

JF Modiano, M Breen, SE Lana, N Ehrhart, SP Fosmire, R Thomas, CM Lubala, AR Laerato-Kozicki, E.J. Ehrhart, J Schaack, RC Duke, GC Cutter, D Bellgrau. Naturally occurring translations models for developoment of cancer gene therapy. Gene Ther Mol Biol. 10; 31-40, 2006.

Siddiqui F, E.J. Ehrhart, Charles B, Chubb L, Li CY, Zhang X, Larue SM, Avery PR, Dewhirst MW, Ullrich RL. Anti-angiogenic effects of interleukin-12 delivered by a novel hyperthermia induced gene construct. Int J Hyperthermia. 2006 Nov;22(7):587-606.

Kraft S, E.J. Ehrhart, Gall D, Klopp L, Gavin P, Tucker R, Bagley R, Kippenes H, DeHaan C, Pedroia V, Partington B, Olby N. Magnetic resonance imaging characteristics of peripheral nerve sheath tumors of the canine brachial plexus in 18 dogs. Vet Radiol Ultrasound. 2007 Jan-Feb;48(1):1-7.

Bush JM, Fredrickson RL, E.J. Ehrhart. Equine osteosarcoma: a series of 8 cases. Vet Pathol. 2007 Mar;44(2):247-9.

Boston SE, Duerr F, Bacon N, Larue S, E.J. Ehrhart, Withrow S. Intraoperative radiation for limb sparing of the distal aspect of the radius without transcarpal plating in five dogs. Vet Surg. 2007 Jun;36(4):314-23.

DiResta GR, Aiken SW, Brown HK, Bergman PJ, Hohenhaus A, E.J. Ehrhart, Baer K, Healey JH. Use of an artificial lymphatic system during carboplatin infusion to improve canine osteosarcoma blood flow and clinical response. Ann Surg Oncol. 2007 Aug;14(8):2411-21. Epub 2007 May 15.

Mangan BG, Al-Yahya K, Chen CT, Gionfriddo JR, Powell CC, Dubielzig RR, E.J. Ehrhart, Madl JE. Retinal pigment epithelial damage, breakdown of the blood-retinal barrier, and retinal inflammation in dogs with primary glaucoma. Vet Ophthalmol. 2007 Nov-Dec;10 Suppl 1:117-24.

J. C. Petty, S. E. Lana, D. H. Thamm, J. B. Charles, A. M. Bachand, J. M. Bush, E. J. Ehrhart. Glucose transporter 1 expression in canine osteosarcoma. Veterinary and Comparative Oncology. 2008, June; 6, (2): 133-140.

J Lawler, M Frye, M Bera, E.J. Ehrhart, J Bright. Third degree atrioventricular block in a horse secondary to rattlesnake envenomation. J Vet Intern Med. 2008 Mar-Apr; 22(2): 486-90

J. P. Farese, N. J. Bacon, N. P. Ehrhart, J. Bush, E. J. Ehrhart, S. J. Withrow. Esophageal leiomyosarcoma in dogs: surgical management and clinical outcome of four cases. Veterinary and Comparative Oncology. 2008 March; 6, (1) 31-38.

Ramos-Vara JA, Kiupel M, Baszler T, Bliven L, Brodersen B, Chelack B, Czub S, Del Piero F, Dial S, E.J. Ehrhart, Graham T, Manning L, Paulsen D, Valli VE, West K; American Association of Veterinary Laboratory Diagnosticians Subcommittee on Standardization of Immunohistochemistry.  Suggested guidelines for immunohistochemical techniques in veterinary diagnostic laboratories. J Vet Diagn Invest. 2008 Jul; 20(4):393-413

Rebhun RB, Lana SE, E.J. Ehrhart, Charles JB, Thamm DH.  Comparative analysis of survivin expression in untreated and relapsed canine lymphoma. J Vet Intern Med. 2008 Jul-Aug; 22(4): 989-95.       

S Disatian, E.J. Ehrhart, S Zimmerman, EC Orton.  Valve Interstitial Cells From Dogs With Natural Degenerative Mitral Valve Disease Undergo Phenotype Transformation. J Heart Valve Dis. 2008 Jul; 17(4):402-11; discussion 412.

J. M. Liptak, D. A. Kamstock, W. S. Dernell, E. J. Ehrhart, S. A. Rizzo, S. J. Withrow. Cranial mediastinal carcinomas in nine dogs. Veterinary and Comparative Oncology. 2008 March; 6(1):19-30.

DH Thamm, JB Charles, YA Elce, E.J. Ehrhart. Cyclooxygenase-2 expression in equine tumors. Vet Pathol. 2008 Nov; 45(6):825-8.
         
Book Chapters:
R. Hamor, E.J. Ehrhart. Eye. In: Textbook of Veterinary Histology, edited by H.D. Dellmann, J. Eurell, 6th edn., Williams and Wilkins, Baltimore, MD.  2006.

E.J. Ehrhart, B.E. Powers. The Pathology of Neoplasia. In: Small Animal Oncology, edited by D.M. Vail and S.J. Withrow, 5th edn., Elsevier, St. Louis, MO. Elsevier Inc, 2007.

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